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The Effect Of Temperature On Beetroot Membranes

736 words - 3 pages

The effect of temperature on the beetroot membranes

Aim of the research: The aim of this investigation is to determine what kind of effect will the increasing temperature have on the plasma membrane of a beetroot cell.

Introduction
The beetroot contains a red pigment that is kept in the cells by the membranes. If the membranes are damaged, the pigment “betalain” will leek out.
The amount of pigment that leeks out can be assessed, as “betalain” will colour any water that surrounds the cell. If the water with the beetroot slices is heated at different temperatures, then will the temperature have any affect on the colour of the solution?

Hypothesis
I think that more red pigment will be released as the temperature increases.
When the beetroots slices in the water are heated, the high temperature will provide more kinetic energy.
The increase in energy will make the molecules inside the beetroots slices to move faster. The fast movement will probably cause damage and break the cell membrane allowing the red pigment to vanish out of the cells.

Temperature (C°) Color of beetroot waters Color intensity(arbitrary units)
0 Faint crème color 0.02
20 Darker crème color 0.02
40 Pink with a red mix 0.06
60 dark red 1.00
80 Orange red .65
100 Yellow/orange .14
Table 1: Observations of the colours of beetroot waters from my data (group 5)

Temperature(C° ± 5.0 C°) Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 Mean SD
0 0,03 0,01 0,13 0,05 0,02 0,00 0,01 0,04 0,04
20 0,04 0,01 0,06 0,00 0,02 0,01 0,00 0,02 0,02
40 0,09 0,05 0,06 0,15 0,06 0,00 0,03 0,06 0,04
60 1,45 0,56 0,90 0,84 1,00 0,05 0,59 0,77 0,40
80 1,45 0,62 2,00 0,65 0,65 0,74 0,87 1,00 0,49
100 0,36 0,21 0,49 0,26 0,14 0,20 0,64 0,33 0,17
Absorbance/ Arbitrary units ± 0.01
Table 2: The table above shows the investigation of beetroot membranes and the results of the absorbance and temperature of the 7 different groups that did the same experiment in order to improve the reliability of the collected data.

Mean

The mean of the data collected for each concentration was calculated in order to compare absorbance in the different temperatures and to reduce the effect of random errors.

Mean= Sum of all data/ Total number of data

E.g. The mean value for 0°C

Sum of all data: 0.03+0.01+0.13+0.05+0.02+0.00+0.01=3.42
Total number of data: 7
Mean: 3.42/7=0.04 (arbitrary...

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