This website uses cookies to ensure you have the best experience. Learn more

The Effect Of H2 O2 Concentration On Catalase Activity

965 words - 4 pages

Introduction
H2O2 is commonly known as hydrogen peroxide and it is a strong oxidizer and a naturally produced compound in humans as a by-product of oxidative metabolism. Because of this, humans also produce the enzyme catalase peroxidases in order to convert small amounts of H2O2 into oxygen and water. It uses the following chemical formula:
2H_2 O_2→O_2+〖2H〗_2 O
This process is called decomposition as two hydrogen peroxide molecules are broken down into oxygen and two water molecules.
Catalase is an important enzyme that protects cells from oxidative damage, which hydrogen peroxide can cause. It is an incredibly efficient enzyme where one catalase molecule can convert millions of hydrogen peroxide molecules each second.
This experiment looked at how substrate concentration can affect enzyme activity. In this case the substrate was hydrogen peroxide and the enzyme was catalase. Pieces of meat providing the catalase were added to increasing concentrations of hydrogen peroxide in order to measure the effect of hydrogen peroxide concentrations on the enzyme’s activity. The variable measured was oxygen produced, as water would be too difficult to measure with basic equipment.
Research Question
What is the effect of the concentration of a hydrogen peroxide solution on catalase activity?
Hypothesis
The hypothesis is that catalase activity will increase exponentially with higher concentrations of hydrogen peroxide until all catalase active sites are filled, in which case the activity will plateau.
Methods
Independent variable: Hydrogen peroxide concentration
Dependent variable: Oxygen production
Controlled variable(s): the surface area of the meat pieces (size and shape were controlled) which affects the concentration of catalase. The same type of meat (liver) and species (cow) was used. All pieces came from the same liver, thereby using only a single animal in the experiment. The temperature of the meat was controlled, aka kept at room temperature. All tries were measured to the same level of oxygen before stopping the timer. The same air syringe was used to measure the oxygen, and it was ensured that the hydrogen peroxide solutions covered the meat pieces during the experiment.
Equipment: Air syringe, cow liver, and 30% hydrogen peroxide
Method:
Prepare hydrogen peroxide solutions using concentrations of 30%, 15%, 7.5%, 3.75%, 1.88%, and 0.94%. Solutions should be 100mL and there should be two of each concentration.
Prepare beef liver by cutting it into similarly shaped and sized rectangles. Attempt to obtain the same relative surface area for each piece.
Set up air syringe and flask. The air syringe should be held horizontally for the experiment, to ensure gravity is not affecting the results. The distance between the flask and the syringe should be close enough that the stopper can be fully pushed into the mouth of the flask.
Begin with the 0.94% solution and work upwards. Pour the solution into the flask and ensure that the stopper is at hand....

Find Another Essay On The Effect of H2O2 Concentration on Catalase Activity

A lab report on the investigation of temperature on catalase activity

828 words - 3 pages INVESTIGATION INTO THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITYAIMThe aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase's different attributes.HYPOTHESISIn this experiment it would be safe to hypothesise that no activity would take place at 1

The Effect Of Substrate on Enzyme concentration

2790 words - 11 pages slope. On the y-axis should be 'Total Volume of O2 collected' vs. x-axis 'Time (seconds)'.The gradient value of the slope of each volume of yeast should then be used to plot another graph showing 'Initial rate of reaction' against 'enzyme concentration'.17. At the end of the experiment the apparatus should betaken apart, and the tubes should be rinsed and then put back into the rack.18. Analyse graphical data19. Draw conclusion20. Evaluate the

Determining the Influence of pH on the Activity of Catalase Enzyme in Potato Tuber Cells

2302 words - 9 pages INTRODUCTIONEnzymes are vitally important for every living organism, because without them the reactions within the cells would occur to slow to sustain life. However there are many factors which affect the rate of enzymes reactions. One of them is pH, which influence on the enzyme activity will be the subject of this investigation. Catalase of potato tuber cells will be the enzyme used in the experiment.RESEARCH QUESTIONWhat is the influence of

Enzyme Activity in the Catalase

2352 words - 9 pages top slowly or not at all. For our temperature experiment we predicated that the hotter or lower the temperature would determine the time it would take to reach the top, and for our pH experiment we predicted that the higher or lower the pH would have an effect on the enzyme activity. Methods: For the Catalase Extraction method, we used approximately 50 g of cut up potatoes, we also added 150mL of cold water then we added it all to the blender

Effect of Concentration on Osmosis

2617 words - 10 pages Effect of Concentration on Osmosis Aim To find out how the concentration of sucrose solution affects the mass of the potato chip left in the solution for one day. Prediction I predict that when the sucrose concentration is low the potato chip will gain mass. This is because there will be a higher sucrose concentration in the potato chip so water will go into the potato from the solution. I predict that when there is a high

Effect of Temperature on Rate of Reaction of Catalase

906 words - 4 pages Effect of Temperature on Rate of Reaction of Catalase Introduction This investigation was in an attempt to try to find out how varying the temperature can affect an enzyme. The enzyme used was catalase which breaks down Hydrogen Peroxide, this gives off water and oxygen as effervescence. This effervescence is what is used to measure the reaction rate of the catalase. The optimal heat for enzyme activity is proven

The Effect of Glucose Concentration on Anaerobic Respiration in Yeast

859 words - 3 pages The Effect of Glucose Concentration on Anaerobic Respiration in YeastIntroduction:Yeast, also known as a saccharomyces cerevisiae, is single celled eukaryotic cells that are in the kingdom fungi and are unicellular organisms which normally reproduce asexually by budding at a very high rate. Scientists quite often decide to work with yeast because of its features fast growing rate and the fact that yeast's DNA can be easily manipulated. Some

The effect of substrate concentrations on the rate of the decomposition of hydrogen peroxide when catalyzed by the enzyme catalase

4245 words - 17 pages For this investigation I have been asked to investigate (by experimentation) the effect of substrate concentrations on the rate of the decomposition of hydrogen peroxide when catalyzed by the enzyme catalase. This is part of our work on the function of enzymes, how they work and the effects of conditions on how they work. We have learnt about the formation of enzyme-substrate complexes, the lock and key model, induced fit model, activation

THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTIVITY

651 words - 3 pages attractions between enzymes. On the other hand, too much salt causes enzymes to denature. Hydrogen peroxide is highly reactive and forms during metabolic reactions that require oxygen. It can also form oxygen radicals that oxidize organic molecules and are potentially quite harmful. The enzyme catalase is able to neutralize hydrogen peroxide and protects the molecules that are important for life. Catalase is an example of an anti-oxidant, aptly named

Transcript of The Effect of Amylase on Starch Concentration - Biology - Assignment

274 words - 2 pages Discussion In conclusion, the results were the same as hypothesized, with higher concentrations of amylase resulting in faster starch breakdown. This can be seen, as the 5% amylase broke down the starch more than the 1% amylase. Sources of error may include incorrect measurements of starch or amylase. Introduction The experiment was conducted in order to find how amylase concentration affects the rate of starch breakdown from its polymer

The effect of concentration on a reaction rate with hydrolic acid over the magnesium chip

2498 words - 10 pages The effect of concentration on a reaction rateAim: In this experiment I have been offered a choice to investigate absolutely anything that I desire to see the diverse effects therefore I have decided to investigate the concentration of the hydrochloric acid with the magnesium chip. I will be altering the concentration of the acid to explore the rates at which it reacts with magnesium, and to see at which concentration more quantity of hydrogen

Similar Essays

The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase

1896 words - 8 pages The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Introduction This is a real A-level school project and as such is intended for educational or research purposes only. Extracts of this project must not be included in any projects

Effect Of Substrate Concentration On Enzyme Activity

1473 words - 6 pages Rate of reaction - volume of O2 released by each concentration of H2O2. Make sure to be at the right eye level with the bottom of the meniscus to read the long graduated tube. Controlled Variables WHY it must be controlled HOW it was controlled 1 Volume of the yeast suspension The experiment was done to investigate is the effect of the substrate concentration on enzyme activity. Always take 5mL of the yeast suspension. 2 Temperature Being at

The Effect Of Level Of P H And Temperature On Catalase Enzyme Activity Over Oxygen Produce Report

1595 words - 7 pages hydrogen peroxide into harmless water and oxygen gas. Dependent variable Catalase enzyme activity (measured as % of oxygen produced) Independent variable 1 experiment: pH 2 experiment: temperature Controlled variable Amount of time The purpose of this lab was to determine the effect of pH and temperature on the rate of catalase enzyme activity. Since the catalase speeds up the

To Determine The Effect Of Substrate Concentration On The Activity Of The Enzyme Amylase. Biology Assignment

1466 words - 6 pages Biology Assessment Task 1 Aim: To determine the effect of substrate concentration on the activity of the enzyme amylase. Hypothesis: As the substrate concentration increases, the activity increases. However after certain concentration of substrate, the activity remains same because of saturation of Active site on Enzyme with substrate Molecules. Materials: · 4 test tubes · test tube rack · 10mL measuring cylinder · stopwatch · 2 50mL beakers