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The Effect Of Hydrogen Peroxide On Catalyse If You Change The Temperature

1465 words - 6 pages

The effect of hydrogen peroxide on catalyse if you change the temperaturePlan:Aim:My aim in this investigation is to find out and investigate how the temperature affects the rates of reaction of the enzyme catalyse on its substrate hydrogen peroxide.An enzyme is a biological catalyst, it alter the rate of reaction without being changed itself. Enzymes are proteins; they have a very precise three-dimensional shape, which forms a one specific active site on the enzyme. Each enzyme can only convert one kind of substrate molecule in to one kind of product molecule. These are specific.What affects Enzymes?· Temperature- Enzymes stop working if the temperature rises above 40ºC. Increasing the temperature alters the 3D shape and so the enzyme can no longer fit the substrate.· pH- They work best in neutral conditions neither acidic nor alkaline.What affect does catalyse have?Catalyse is a very fast reacting enzyme, it is found in many living cells, it breaks down hydrogen peroxide to water and oxygen. In fact one molecule of it can deal with six million molecules of hydrogen peroxide in 1 minute. Hydrogen peroxide is toxic so needs to be changed into harmless substances.Apparatus:· Hydrogen peroxide 30ml· 25ml cylinder tube· Kettle· Water bath· Hot water bath· Delivery tube· Conical flask· Thermometer· Clamp· Burette (60cm)· Stop watch· White tile· Knife· Ruler· Rubber bung· Goggles· Flask· Stop watch· PotatoMethod:· Put goggles on· Gather all the equipment· I will then put all my apparatus together ensuring that I have assembled everything correctly· Warm the water up in the kettle· I will then get the white tile and place the potato onto the tile· While the water is warming up, using the cork borer, (6cm) I will make 5 cylinders from the large potato, cutting them all accurately so they are the same length, measuring them accurately with a ruler(1cm each)· Measure 25ml of hydrogen peroxide using a measuring cylinder.· Select the temperature you are going to study0ºC- iced water25ºC-no extra equipment37ºC-water bath required55ºC-water bath required100ºC-beaker of boiling water· Fill up one of the water baths with normal tap water-about half way· Then I will fill the burette with water using a funnel· Add the measured hydrogen peroxide of 20 ml, and attach the rubber bung connected to the measuring syringe.· While holding one end of the burette, run it and attach it to the clamp and place it into the water bath.· Then fill in the 2nd water bath with the water that has been warmed up in the kettle.· I will measure the temperature of the water using a thermometer and ensure that the temperature of the water is constant.· Then attach the end of the delivery tube to the end of the...

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