This website uses cookies to ensure you have the best experience. Learn more

The Effect Of Substrate On Enzyme Concentration

2790 words - 11 pages

"h Wear goggles, apron and gloves to be safe in case of spillage or accidental contact with clothing."h Upon contact with solution it is advisable to wash hands or affected body part immediately. Be careful as it burns."h If breakage of tubes/beaker occurs, clean up immediately."h In order not to confuse test tubes containing water and hydrogen peroxide, label each of them clearly"h Remember to wash hands after handling yeast suspensionFAIR TESTTo ensure that a fair test is carried out the following must be observed"h Make sure fixed variables are not altered"h Make sure that when the volume of yeast and water is changed and then added to hydrogen peroxide the total volume is 8 cm3."h Make sure the volume of hydrogen peroxide in all the experiments is kept constant at 3 cm3"h Ensure the clamp is firmly in place so there are mishaps and no interruptions"h Carry out experiment more than once and then find an average this should eliminate anomalies"h Mix the reagents and start the clock at the same time, so that the correct value will be got."h Make sure air bubbles aren't formed before the reaction begins by using the bung"h Try as much as possible to avoid parallax error.FINAL CHOICE OF EQUIPMENTThe set up of my apparatus will be similar to my preliminary experiment, just a few changes have been made and a few things added:"h Clamp (x1) (indicated by arrow)"h Metal retort stand (x1)"h Electronic timer (recording to 1 sec intervals) (x1)"h Beaker (500 cm3) (x1)"h Micro test tube to fit into side-arm boiling tube"h Large side-arm boiling tube and bung fitted with delivery tube to fit (x5)"h Graduated measuring cylinder (10 cm3) (x2)"h Graduated pipette (5/10 cm3) and safety filler (x2)"h Gloves"h Apron"h Goggles"h Test tube rack (allow 2)"h Forceps"h Gas buretteREAGENTS"h Tap water"h 1% (w/v) yeast suspension (freshly prepared)"h 5 vol. hydrogen peroxideTECHNIQUES EMPLOYEDParallax errors were avoided by looking at bottom the meniscus of water in the gas burette.DETAILED PROCEDURE1. Put on apron, gloves and goggles2. Before hand ¡V using a pipette measure out 1 cm3 of yeast suspension and use the measuring cylinder to measure 4 cm3 of water, and then transfer both reagents into the side ¡Varm boiling tube number 1."h Do the same thing for 2 cm3 of yeast and 3 cm3 of water. Keep doing this consecutively till the volume of yeast is 5 cm3 and water is 0 cm3."h Label the side ¡V arm boiling tubes 1-5 and also state their contents i.e. 1 cm3 and 4 cm3 2H2O2.Place all of then in the rack"h Then measure out using the 2nd pipette into 5 micro test tubes, 3 cm3 of hydrogen peroxide, and these tubes don't have to be labelled as it can be distinguished from the rest. The out come should look like this:YeastHydrogen PeroxideWater Total vol. Of Reagents(cm3)(cm3)(cm3)(cm3)134823383328431853083. Set up apparatus as shown in the diagram4. Take the first side-arm boiling tube, i.e. 1cm3 yeast + 4cm3 water and connect to the stand using the clamp....

Find Another Essay On The Effect Of Substrate on Enzyme concentration

Effects of Enzyme Rate by Variables of Enzyme Concentration, Substrate Concentraion, Temperature and pH

3910 words - 16 pages then be used to take absorbance numbers, calculate concentration PNP and thus rate of reaction. The linear relationship equation is (absorbance) = .0172[p-nitrophenol] - .00250 or otherwise written as [p-nitrophenol] = 58.1395 x (absorbance) + .1453. For calculations of obtaining te standard curve, refer to Appendix 1.We then tested the effect of increasing the amount of enzyme on the rate of production of PNP. We began our experiment with 6

To investigate the effect of surface area on the rate of reaction using the enzyme Catalase, found in potato, and the substrate Hydrogen Peroxide

2672 words - 11 pages substrates are bonded to make one product causing an anabolic reaction.There are five factors that effect enzyme activity. They are:·Temperature·pH·Inhibitors·Substrate Concentration·Enzyme ConcentrationThe factor I am changing is surface area of potato containing Catalase. By increasing the surface area of the potato, you make more Catalase molecules be exposed to hydrogen peroxide. This will result in the rate of

To investigate the effect of surface area on the rate of reaction using the enzyme Catalase, found in potato, and the substrate Hydrogen Peroxide

541 words - 2 pages Hydrogen peroxide has an acidic pH which will break down into water which has a neutral pH. This may effect the enzyme action as many enzymes work faster in a neutral environment, the only control over this is the fact that it will happen in each test and to consider its effect at the end of the experiment. I will need to use the same potato and cut a fresh chip at each stage of the experiment as the catalase may become effected by exposure to

What affect the substrate has on the rate of reaction with the enzyme in potato?

946 words - 4 pages Enzyme InvestigationTaskTo find out what affect the substrate has on the rate of reaction with the enzyme in potato.Variables1. PH2. Temperature3. Concentration of substrate4. Concentration of enzyme5. Surface area of potato lumps6. TimeKey VariablesInput variables - concentration of substrateOutput variable - volume of gasPredictionI think that to start with, the higher the concentration of the

THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTIVITY

651 words - 3 pages helping the substrates to bind together. It is easier for substrates to react if they are closer together, thus bringing on the transition state. The second way is by repositioning them. Substrates do not travel in a particular direction and so if they are repositioned so that they collide, they will react. Another way to cause reactions is using an induced-fit model. This means that the enzyme changes its shape to better fit the substrate and make

Biology: Experiment- The Effect of Temperature on the Enzyme Rennin

1890 words - 8 pages Aim: The aim of the experiment is to test the effect temperature has on the activity of the enzyme rennin.Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC, which is the body temperature, where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the

Effect of Concentration on Osmosis

2617 words - 10 pages Effect of Concentration on Osmosis Aim To find out how the concentration of sucrose solution affects the mass of the potato chip left in the solution for one day. Prediction I predict that when the sucrose concentration is low the potato chip will gain mass. This is because there will be a higher sucrose concentration in the potato chip so water will go into the potato from the solution. I predict that when there is a high

The Effect of H2O2 Concentration on Catalase Activity

965 words - 4 pages down into oxygen and two water molecules. Catalase is an important enzyme that protects cells from oxidative damage, which hydrogen peroxide can cause. It is an incredibly efficient enzyme where one catalase molecule can convert millions of hydrogen peroxide molecules each second. This experiment looked at how substrate concentration can affect enzyme activity. In this case the substrate was hydrogen peroxide and the enzyme was catalase. Pieces

The Effect of Glucose Concentration on Anaerobic Respiration in Yeast

859 words - 3 pages The Effect of Glucose Concentration on Anaerobic Respiration in YeastIntroduction:Yeast, also known as a saccharomyces cerevisiae, is single celled eukaryotic cells that are in the kingdom fungi and are unicellular organisms which normally reproduce asexually by budding at a very high rate. Scientists quite often decide to work with yeast because of its features fast growing rate and the fact that yeast's DNA can be easily manipulated. Some

Investigating the effect of temperature on the action of a protease enzyme on exposed developed film

2951 words - 12 pages the activity of enzymes they are:"Y Temperature"Y pH"Y Specificity"Y Concentration of enzyme or substrateEnzymes are specific, this means that they only work on one substrate molecule. A substrate molecule is what the enzyme actually works on.The factors I have chosen to investigate are temperature. This therefore means that the temperature will be the independent variable.In the experiment there will be a transparent plastic backing of developed

An Investigation Into The Effect Of Enzyme

2633 words - 11 pages visit http://www.iizukakei.com for free bogusmail.fake mail and book reviews and more! An Investigation Into The Effect Of Enzyme Concentration On The Rate Of Catalase Activity Equation: 2H2O 2 2H2O + O 2 Catalase is used to catalyse this reaction as hydrogen peroxide takes too long to decompose on its own. The source of catalase which I will be using will be from potato.Hypothesis: I believe that as the concentration of enzyme is increased, the

Similar Essays

Effect Of Substrate Concentration On Enzyme Activity

1473 words - 6 pages Background An enzyme is a biological catalyst which speeds up biochemical reactions, such as digestion and respiration, but they remained unchanged at the end of the process (Walpole, Merson-Davies, and Dann 53). Problem Question What is the effect of the substrate concentration on enzyme activity? Hypothesis As the concentration of the substrate increases, the rate of reaction also increases until it reaches its maximum point where all

The Effect Of Concentration Of Enzyme And Enzyme Substrate On The Rate Of An Enzyme Catalysed Reaction

3271 words - 13 pages decrease due to denaturation of the enzyme.I would expect from this reaction as the concentration of the enzyme catalase is increased that the amount of oxygen produced would increase, too. This is because catalase speeds up the decomposition of hydrogen peroxide into water and oxygen. Catalase exerts the effect on chemical reactions by providing a place for the hydrogen peroxide, the substrate, to interact. So as the concentration of catalase increases

The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase

1896 words - 8 pages The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Introduction This is a real A-level school project and as such is intended for educational or research purposes only. Extracts of this project must not be included in any projects

The Effect Of Substrate Concentrations On The Rate Of The Decomposition Of Hydrogen Peroxide When Catalyzed By The Enzyme Catalase

4245 words - 17 pages For this investigation I have been asked to investigate (by experimentation) the effect of substrate concentrations on the rate of the decomposition of hydrogen peroxide when catalyzed by the enzyme catalase. This is part of our work on the function of enzymes, how they work and the effects of conditions on how they work. We have learnt about the formation of enzyme-substrate complexes, the lock and key model, induced fit model, activation