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The Effect Of Substrate On Enzyme Concentration

2790 words - 11 pages

"h Wear goggles, apron and gloves to be safe in case of spillage or accidental contact with clothing."h Upon contact with solution it is advisable to wash hands or affected body part immediately. Be careful as it burns."h If breakage of tubes/beaker occurs, clean up immediately."h In order not to confuse test tubes containing water and hydrogen peroxide, label each of them clearly"h Remember to wash hands after handling yeast suspensionFAIR TESTTo ensure that a fair test is carried out the following must be observed"h Make sure fixed variables are not altered"h Make sure that when the volume of yeast and water is changed and then added to hydrogen peroxide the total volume is 8 cm3."h Make sure the volume of hydrogen peroxide in all the experiments is kept constant at 3 cm3"h Ensure the clamp is firmly in place so there are mishaps and no interruptions"h Carry out experiment more than once and then find an average this should eliminate anomalies"h Mix the reagents and start the clock at the same time, so that the correct value will be got."h Make sure air bubbles aren't formed before the reaction begins by using the bung"h Try as much as possible to avoid parallax error.FINAL CHOICE OF EQUIPMENTThe set up of my apparatus will be similar to my preliminary experiment, just a few changes have been made and a few things added:"h Clamp (x1) (indicated by arrow)"h Metal retort stand (x1)"h Electronic timer (recording to 1 sec intervals) (x1)"h Beaker (500 cm3) (x1)"h Micro test tube to fit into side-arm boiling tube"h Large side-arm boiling tube and bung fitted with delivery tube to fit (x5)"h Graduated measuring cylinder (10 cm3) (x2)"h Graduated pipette (5/10 cm3) and safety filler (x2)"h Gloves"h Apron"h Goggles"h Test tube rack (allow 2)"h Forceps"h Gas buretteREAGENTS"h Tap water"h 1% (w/v) yeast suspension (freshly prepared)"h 5 vol. hydrogen peroxideTECHNIQUES EMPLOYEDParallax errors were avoided by looking at bottom the meniscus of water in the gas burette.DETAILED PROCEDURE1. Put on apron, gloves and goggles2. Before hand ¡V using a pipette measure out 1 cm3 of yeast suspension and use the measuring cylinder to measure 4 cm3 of water, and then transfer both reagents into the side ¡Varm boiling tube number 1."h Do the same thing for 2 cm3 of yeast and 3 cm3 of water. Keep doing this consecutively till the volume of yeast is 5 cm3 and water is 0 cm3."h Label the side ¡V arm boiling tubes 1-5 and also state their contents i.e. 1 cm3 and 4 cm3 2H2O2.Place all of then in the rack"h Then measure out using the 2nd pipette into 5 micro test tubes, 3 cm3 of hydrogen peroxide, and these tubes don't have to be labelled as it can be distinguished from the rest. The out come should look like this:YeastHydrogen PeroxideWater Total vol. Of Reagents(cm3)(cm3)(cm3)(cm3)134823383328431853083. Set up apparatus as shown in the diagram4. Take the first side-arm boiling tube, i.e. 1cm3 yeast + 4cm3 water and connect to the stand using the clamp....

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