This Is An Experiment On How Planaria Regenerate Body Parts And An Explaination As To How The Experiment Was Performed.

1473 words - 6 pages

AbstractThis experiment was undertaken to evaluate the varying degrees and limitations of regeneration using flatworms. Regeneration occurs in many levels throughout the animal kingdom. The flatworms demonstrated substantial healing abilities once the surgery had been performed. Total regeneration and reproduction had occurred resulting in three separate flatworms from one that had been cut through three quarters of the length of the body.IntroductionPlanaria belong to the phylum Platyhelminthes (flatworms). They are free-living, flat bodied, freshwater animals that demonstrate the notable ability to regenerate their lost body parts. "In some genera, when a single individual is divided into two or more parts, each part can regenerate an entirely new flatworm" (Johnson). In addition to reproducing asexually by regeneration, the hermaphroditic planaria feature an internal reproductive system. When two flatworms join, each exchanges sperm and fertilizes the other's eggs. Their simple body plan also allows them to regenerate complete bodies from mere fragments by asexual reproduction. Their body plan consist of a simple nervous system that includes a primitive brain, central nervous system, muscles, an internal reproductive system, intestines and an excretory system that includes specialized flame cells. In this experiment, a flatworm was used to assess the regeneration process over the course of three weeks.Materials and Methods·Remove ice container from freezer, snap it shut and place on the stage of a dissecting microscope.·With a camel's hair brush carefully place one flatworm on the center of the ice container surface.·Wait until the animal has flattened out.·Use a clean, sharp razor blade to make a quick cut from the tip of the head area (between the eyespots) through three-quarters the length of the flatworms body allowing the pharynx to go to one side or the other.·Cut will have to be renewed every twelve hours for the first three days.·Rinse the flatworm from the ice block, with holding solution from a dropper, into a petri dish.·Fill petri dish half way with the holding solution.·Place the petri dish in a cool, dark container for the duration of the experiment.·Holding solution must be replaced twice each week.·Discard dead white worms when replacing holding solution or after the flatworms have eaten.·About one month is required to complete regeneration.Results:Day 1: (09/18/03)Cut between eyespots three quarters of the length of the body.Pharynx could not be mediated; so, it went to one side of the body.Figure 1Day 1-Day 3: (09/18/03 - 09/21/03)Checked every twelve hours to ensure incision had not grown backOn 09/19/03, one-third of the incision had grown back together. Recut.Figure 2Day 7: (09/25/03)First observation reflecting dicephalization.Two distinct heads joined above single pharynx where incision had mendedEach head had two distinct auricles and two...

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