Tim The Tool Essay

521 words - 2 pages

Determining Amino Acids by Chromatographic Techniques By Big Jim The first step in determining the amino acid sequence of our peptide was to find the length of the peptide by a process called titration. Basically this process in tales pouring a certain amount of both phenolphthalein and formalin into a flask, then NaOH is added by an eyedropper until the solution turns a pale pink. You do this same procedure to three different flasks; however, a different chemical is added to each one. In one of them the formalin solution is added, to another add a certain amount of the unhydrolyzed peptide, and then in the last one add a certain amount of the hydrolyzed peptide solution. To all of these flasks more NaOH is add until the solutions turn pale pink and then the volume is recorded. These volumes are used to calculate the ratio of carboxylic groups from the hydrolyzed and unhydrolyzed peptide solutions. This ratio tells the length of the peptide chain, and if the experiment is conducted correctly the length should be three, meaning that this is a tripeptide.After we determined how long the peptide was we needed to find what amino acids that made up the peptide. To do this we used cellulose strips. We measured 1.5 cm from one end of the cellulose strip. This was where we would apply the amino acid that we wanted to test. We would apply a small drop using a capillary tube, dry it, then add another drop; this was repeated three times. After this was completed we placed the...

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