To Determine The Effect Of Substrate Concentration On The Activity Of The Enzyme Amylase. Biology Assignment

1466 words - 6 pages

Biology Assessment Task 1
Aim:
To determine the effect of substrate concentration on the activity of the enzyme amylase.
Hypothesis:
As the substrate concentration increases, the activity increases. However after certain concentration of substrate, the activity remains same because of saturation of Active site on Enzyme with substrate Molecules.
Materials:
· 4 test tubes
· test tube rack
· 10mL measuring cylinder
· stopwatch
· 2 50mL beakers
· disposable pipettes
· iodine solution (substrate concentration)
· amylase solution (enzyme)
The following will be available in Stock bottles for collection as required:
· 1% starch solution (reactant)
· 10% starch solution
· 20% starch solution
· 30% starch solution
Method:
(BASED OFF PEERS) (DO THIS ONE)
1. Collect all equipment
2. Label test tubes 1 - 5
3. Using a measuring cylinder, measure 10 ml of starch
4. , In test tube 1. place 10 ml of 1% starch
5. In test tube 2. place 10 ml of 10% starch
6. In test tube 3, place 10 ml of 20% starch
7. In test tube 4 place 10 ml of 30% starch
8. In test tube 5, don't place any starch solution (control) (Need to complete steps 9-10 for test tube 1-4, first before continuing to test tube 5, as you’ll need to wash the other test tubes
9. Then in each test tubes place 10 ml of iodine solution
10. At one at a time place 10 ml of amylase solution and record how long using a stopwatch, it takes for the reactant to react or change from solution
11. Repeat experiment twice/or (label tests tube 6-10 and repeat)
12. Graph/Tabulate results
OR (BASED OFF PART A)
1. Collect all equipment
2. Label test tubes 1 - 4
3. In test tube 1, 2, 3, 4. place 10 ml of 10 ml of Amylase solution
4. Then in each test tubes 3 and 4 place 10 ml of starch 1% solution, leaving 1, 2 as controls
5. At one at a time place 10 ml of iodine solution and record how long it takes for the reactant to react or change colour
6. Wash all equipment, then repeat experiment for 10%, 20% and 30% starch, relabeling each test tube 5-8(5-6 control) (10%), 9-12 (9-10 control) (20%) and 13-16 (13-14 control) (30%)
7. Graph/Tabulate results
OR (BASED OFF PART B)
1. Collect all equipment
2. Label test tubes 1 - 4
3. In test tubes 1 - 4 place 10 ml of 1% starch
4. In test tubes 3 - 4 place 10 ml of Amylase solution leaving test tubes 1 - 2 as controls
5. In all test tubes place 10 ml of Iodine solution and record how long it takes to change colour
6. Repeat steps 4-6, but this time with 10%, 20% and 30%
7. Record all results and graph
OR (BASED OFF PART C)
1. Collect all equipment
2. Label test tubes 1 - 4
3. In test tube 1, 2, 3, 4. Place 10 ml of amylase solution
4. In test tube 1, place 10 ml of Starch solution
5. In test tube 2, place 7.5 ml of Starch solution and 2.5 ml of H20
6. In test tube 3, place 5.0 ml of Starch solution and 5.0 ml of H20
7. In test tube 4, place 2.5 ml of Starch solution and 7.5 ml of H20
· In test tube 5, place 0 ml and 10 ml of H20 of Starch solution (No need to do just for Method)
8. In...

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