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To Find Out The Optimum P H At Which The Enzyme Catalase Would Best Break Down Hydrogen Peroxide.

2844 words - 11 pages

GCSE SCIENCE COURSEWORK (BIOLOGY)PLANNING: Skill Area PWrite down the aim of your Investigation.The aim of investigation is that to find out the optimum pH at which the enzyme catalase would best break down hydrogen peroxide.Briefly describe your Preliminary Experiment and include your results. Explain how these results have helped you plan your main experiment (p8b).The purpose of the preliminary experiment was to find out the best source of catalase for the investigation. The four choices of sources which were available were liver, apple, yeast and potato. Each source was cut up into 1cm3 sections and placed in four separate test tubes which each contained 5ml of H2O2. Observations were made on the physical evidence of what happened.Catalase source Degree of bubbling Number of timesrelights glowing splintpotato normal 1apple little 0liver a lot 7yeast Quite a lot 3As the result show above, the potato was considered to be the best source to be used in the real experiment because it produced enough oxygen (but not too much to cause a dangerous situation). The apple did not appear to have sufficient catalase for the experiment as it did not produce any oxygen. The yeast and the liver, while rich in catalase unfortunately proved to be over sufficient with an unnecessarily large supply of the enzyme needed. The oxygen is produced by this equation:List all the apparatus that you now plan to use (diagrams should be included) (P4b).20ml of each buffer solution from pH3 to pH8120ml of H2O2 6 test tubesThree medium sized raw potatoes one cork borer50ml Manometer fluid 2x syringes1x Ruler 2x Pipettes1x White tile 1x stopwatch1x scalpel 2x measuring cylinder1x clamp stand 1x glass pencilDescribe the method you plan to use, include all safety precautions (p4a)SafetyHydrogen peroxide is irritable to the human skin so care must be taken to avoid direct contact with the solution.Laboratory coats were worn during the investigation to prevent chemicals from spoiling clothes.Whilst using the razor blades, care was also taken to hold them by the handle and not the blade to prevent an accident occurringAll apparatus was kept away from the edge of the bench so that the possibility of any equipment being knocked over was reduced.A sense of mutual respect should be observed at all times to ensure that a sensible code of conduct was maintained. This included no running about, no sudden activity and no raised voices.Method1. A borer was used to extract equal sized cylindrical shapes from the potatoes. These were sectioned into 1cm3 cylinders with a scalpel.2. 5 potato pieces were placed in the test tube3. And 5ml of buffer solution was then added to the potato pieces.4. 5ml of hydrogen peroxide was then poured into the test tube.5. The mouth of the test tube was closed tightly with the bung through the use of a clip.6. The time was then measured as to how fast the manometer fluid in the manometer tube rose up to the 5cm-line mark which had been drawn on.7. This...

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