This website uses cookies to ensure you have the best experience. Learn more

Topic: Cell Free Plasma Dna As A Predictor Of Outcome In Severe Sepsis And Septic Shock

555 words - 2 pages

Introduction: The research is a clinical and correlation experiment performed by a Finland study group to find out the effect of increased concentration of cell free DNA found in 255 patients 18yrs or older who were seriously ill with whole body inflammatory state in both Intensive care unit and hospital mortality, as well as to see if there was a relation between cell free plasma DNA and organ failure.Procedure:Real time quantitative PCR assay for Beta globin gene was used to measure blood samples Age: 18yrs and older patients with severe sepsis or septic shock.Time: 72hrs after their blood was collected over a period of 4months in 24hrsInstrument: Centrifugation was used to separate the plasma fraction and the blood samples.Storage: stored at -20 degree C and later sent to Helsinki University Hospital, where they were stored at -80 degree C.Data Collection: Simplified acute physiologic score (SAPS II), acute physiology and chronic health evaluation (APACHE II), Sequential organ failure assessment (SOFA), lactate concentration and creatine clearance score using Cockcroft Gault formula were used to study the amount of cell free plasma DNA in survivors and non survivors in both ICU (intensive care unit) and Hospital patients.METHODS:1. The QIA amo DNA Blood Mini Kit (Qiagen) was used to extract DNA in order to ensure there weren’t any residual cells left behind.2. The DNA extracted produced the following sequence: forward primer 5’-GCA CCT….GAA-3’. Reverse primer 5’CAC CAA……TCA-3’ as well as a single labeled fluorescent MGB probe 5’-FAM-TCT….MGB-NFQ. (Minor grove binding molecule and Nonfluorescent quencher molecule respectively).Precision of Data:The data was ran 8times in multiple duplicates to ensure precision of the real time quantitative PCR...

Find Another Essay On Topic: Cell Free Plasma DNA as a Predictor of Outcome in Severe Sepsis and Septic Shock

Predictor Variables Of Teenage Pregnancy And Effectiveness Of Prevention Programs

2956 words - 12 pages research report highlights these trends of indicators or teenage pregnancy in addition to the effectiveness of a variety of different pregnancy prevention program.Young, Martin, Young and Ting (2001) applied two theory's regarding teenage girl's self-perception: Bandura's (1994) theory of self-efficacy and Rotter's concept of locus of control. Self-efficacy is defined as a person's beliefs about his or her ability to attain particular goals. It has

Satire, Humor, and Shock Value in Swifts' A Modest Proposal

1765 words - 7 pages Satire, Humor, and Shock Value in Swifts' A Modest Proposal Swift's message to the English government in "A Modest Proposal" deals with the disgusting state of the English-Irish common people. Swift, as the narrator expresses pity for the poor and oppressed, while maintaining his social status far above them. The poor and oppressed that he refers to are Catholics, peasants, and the poor homeless men, women, and children of

Confirmation of Bacterial Transformation and DNA as the Inducing Material

1204 words - 5 pages Confirmation of Bacterial Transformation and DNA as the Inducing Material Biol2000Lab5: Randall Barley April 15 2014 (late) 001167698 Julius Moore   Confirmation of Transformation and DNA as the Inducing Material Introduction The experiments in this report are a recreation the famous Griffith and Avery experiments which discovered the transformation process that bacteria can undertake and that DNA is the genetic material, respectively

Filmmaker's Use of Shock in Psycho and Jaws

1835 words - 8 pages Shock is a feeling of fascination and excitement mixed with anxiety, tension, suspense and surprise developed from an unpredictable, mysterious, and engaging source of entertainment (Merriam-Webster). Shock can occur whenever there is a perceived suspended drama, with tension, suspense or surprise being the primary emotions felt by the audience as part of the situation (Dirks). However, the term is most often used in regards to an audience’s

Topic: The uncertain future of English as a global language

1314 words - 6 pages Topic: The uncertain future of English as a global language Rationale My written task is based on part one of the English course (Language in cultural context). I have chosen to do an article based on the future of English as a global language. I chose to do this because English today is gaining popularity worldwide, in certain cases influencing the syntax of other languages. In class we spent time discussing the effects of use of English

A discussion of Margerat Atwood's use of shock in 'The Handmaid's Tale, and how it relates to current issues

2649 words - 11 pages incriminating anyone. She recognises herself as a coward, and this almost neutralises the compassion we feel for her plight, and makes us question her viewpoint and level of indoctrination.The shock of possibility is most obviously spelt out in the Historical Notes on the Text, when the circumstances of the rise of Gilead are given a more factual basis, though this understandably vague. '[It was] an age of plummeting Caucasian birth rates' is the reason

A Study in DNA

688 words - 3 pages , one can become part of a rung of a bridge that is trying to reduce the distance between the unknown and the known. Genetics is interesting to me because it provides an immense opportunity for me to alleviate suffering of millions of people by participating in the crusade of thousands of diseases.By first decoding human genome in much more detail like the exemplatory human genome project, I would start decoding the wealth of information stored in

Accuracy of Bayley Scores as Outcome Measures in Trials of Neonatal Therapies? A Central Review of Examiner Errors During the 18 Mo Follow-Up of t

1260 words - 6 pages infants in the first three years of life (Luttikhuizen) and is a common outcome measure used in clinical research trials. Standardized developmental assessments such as the Bayley that are used in a pediatric clinical trial setting as primary outcome measures need to be reliably administered and scored. Trained test administrators and scorers are generally careful that the tests they are administering are conducted and scored correctly. However

CO2 Emission as a severe Global Environmental Concern

1359 words - 6 pages effort has provided multiple ideas including those of chemists, professors, and world corporations. The efforts provided by these group of people of improving certain energy producing methods such as Nuclear energy plants offers hope to ultimately reduce the globe’s high emission of CO2, thus seizing the climb of global temperatures. Proven a key environmental topic in 205 Easy Ways to Save The Earth by Thomas Friedman, the author spends a great

"The Critic" Twain, a severe critic of the general public and its cruel, inane conventions, ridicules religion, lower class, and upper class, in "The Adventures of Huckleberry Finn."

642 words - 3 pages The CriticMark Twain's novel, "The Adventures of Huckleberry Finn," pokes fun at society on all different levels. From dialect and word usage to religion and people's actions, many aspects of society are satirized. This criticism makes the drama in the book light instead of heavy. Twain, a severe critic of the general public and its cruel, inane conventions, ridicules the following three aspects of society; religion, the lower class, as well as

How was the development of specialized compartments in the eukaryotic cell advantageous and what problems needed to be overcome as a result?

749 words - 3 pages cells because they act within the cytosol for roles such as the first stage of the breakdown of sugar. However the bound ribosomes are needed for specialised proteins that will function within organelles. The Golgi apparatus then modifies the products of the ER and transports them in vesicles to their required destinations within the cell or to the plasma membrane to be released outside the cell (Cooper 2000). Another essential part of a cell

Similar Essays

Detection And Treatment Of Patients With Severe Sepsis

2519 words - 10 pages , 2010; Medsafe, 2011). Lastly, administration of high-flow oxygen is cited in a number of studies as a treatment intervention for patients in septic shock; however, there is limited evidence of the benefits on patient outcome following oxygen delivery in the absence of hypoxia (Branson & Johannigman, 2013; Guerra et al., 2013; Perman, Goyal, & Gaieski, 2012). The treatment of the sepsis cascade is a dynamic process and the key red flags in patient

Childhood Obesity As A Predictor Of Premature Death

917 words - 4 pages poisoning, drug overdose, and alcoholic liver disease (Rabin, 2010). The study concluded that as stated by Rabin (2010), ‘Adults who had the highest body mass index scores as children were 2.3 times as likely to have died early as those with the lowest scores, and those with the highest glucose levels were 73% more likely to have died prematurely’ (para. 8). The study also concluded that high blood pressure in childhood was only a weak predictor of

Sepsis: Early Detection And Implementation Of Sepsis Resuscitation Bundle

1265 words - 6 pages at the bedside 24 hours a day they can greatly impact patient outcomes and decrease the overall mortality rate of sepsis by using the evidence-based practices outlined in the Surviving Sepsis Campaign. Reference Page Bernstein, M., & Lynn, S. (2013). Helping patients survive sepsis. American Nurse Today, 8(1), 24-28. Retrieved from http://www.americannursetoday.com/article. Gobel, B., & Peterson, G. J. (2010). Sepsis and septic shock. Clinical

The Subject Of Free Will As Shown In Kafka's "The Judgement", "A Report To The Academy", And "A Country Doctor"

687 words - 3 pages events are inevitable consequences of antecedent sufficient causes; often understood as denying the possibility of free will," a belief that is clearly shown in all of his works, including the three short stories of "The Judgement," "A Country Doctor," and "A Report to the Academy."