In conclusion, the results were the same as hypothesized, with higher concentrations of amylase resulting in faster starch breakdown. This can be seen, as the 5% amylase broke down the starch more than the 1% amylase. Sources of error may include incorrect measurements of starch or amylase.
The experiment was conducted in order to find how amylase concentration affects the rate of starch breakdown from its polymer state to its monomer (glucose) state. The rate of break down of starch by the amylase should determine how differing concentration affects the rate of hydrolysis. This was carried out by combining varied amylase concentrations with starch.
1. Measure 5 mL of starch with pipette and add into 4 test tubes.
2. Add 3 drops of iodine to each and mix
3. Label the other test tubes 1,2,3,4,and 5. Add 5 mL of 1% amylase to tube 1.
4. Mix and record the colour change.
5. Repeat step 3 with all test tubes using the 3%...