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Unidentified Essay

828 words - 4 pages

After much thought, I chose my original sample from my son’s computer mouse. I figured it would be a great location where many different types of bacteria could exist. Fifteen year old boys are not the most hygienic creatures and this is why I swabbed the mouse. However, during the beginning stages of the identification process, my sample turned out to be a fungus. My only option was to then use the sample that my wife had taken off her work shoe. She is a nurse at a hospital and is exposed to many different environments. She swabbed the side of her shoe that she wore for the first time to work. I then started the biological testing on my new sample.
The first test we did was the ...view middle of the document...

Hydrogen peroxide is a byproduct of aerobic respiration. Bubbles were immediately seen, giving a positive result, meaning the bacteria can neutralize H2O2 using the enzyme catalase. We then moved to the starch plate. After inoculating the plate with the bacteria, it needed to be placed into the incubator for forty eight to seventy two hours. We then placed iodine onto the plate and waited for the results. It needed to soak for up to thirty minutes before reading the results. When Iodine reacts with the starches on the plate, it will turn a bluish black color. After incubation, you are looking for the absence of starch around the lines of bacteria. There was no clear zone around the bacteria, which was a negative result. One of the last tests we preformed was the disk-diffusion method. We inoculated a plate, and then placed different types of antibiotic disks onto the plate. If a clear zone is present around the disk, the antibiotic or testing agent is more effective. If the bacteria were touching the disk, the compound was ineffective. My bacteria were sensitive to both Tetracycline and Optochin.
Corynebacterium xerosis is a bacterium that is commonly found in the soil, nature, plants, animals and humans. It is often found on the skin and mucous membranes of human skin. They are Gram-positive and have a rod shaped bacteria. Most corynebacteria do not cause disease and are...

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