BIOLOGY CORE PRATICAL – USING ASEPTIC TECNIQUES TO INVESTIGATE THE EFFECT OF ANTIMICROBIAL SUBSTANCES OF MICROBIAL GROWTH
1) Antibacterial hand wash – to wash hands to kill any bacteria on the surface.
2) Tissues – to wipe hands and to hold the beaker while transferring agar gel in to the agar plate.
3) Gloves & goggles – to prevent the transfer of bacteria form hands and to protect bacteria entering our eye.
4) Sterile agar plate x2 – to place warm agar gel and allow it to settle in sterile condition. (one plate is contaminated with E-coli bacteria)
5) Sterile E. coli culture solution – used to observe the effect of antimicrobial substance on the growth of microbes.
6) Sterile pipette – to transfer E-coli solution in to the agar gel to contaminate it.
7) Sterile incubation loop/spreader – used to transfer and spread E-coli bacteria evenly on to the surface of the agar.
8) Antibiotic discs /paper disk– contains anti-bacterial substances with are then placed into the contaminated agar plate. (ethanol, penicillin and sulphafurazole (SFZ) was also used)
9) Industrial disinfectant – special disinfectants that are targeted to kill majority of the bacteria. This ensures that bacteria from elsewhere doesn’t interfere.
10) Beakers x2 – one used to separate the disinfectant in to needed amounts and another used to make agar gel.
11) Cotton wool – soaked onto the industrial disinfectant in order to disinfect the working area.
12) Sterile tweezers – to lift the cotton wool from the E-coli containing flask. Also used to pick up antibacterial discs.
13) Beaker of ethanol – an alcohol used as disinfectant to kill bacteria. Metal equipment such as the tweezers and spreader are often dipped into ethanol and heated to sterilise them.
14) Thermometer – to measure the temperature of agar solution.
15) Bunsen burner – used to heat agar solution to 93o C and use a blue flame to heat equipment to make them sterile (some equipment are first dipped into ethanol and then flamed)
16) Heat proof mat – placed onto the tripod to prevent the equipment from overheating.
17) Tripod – to hold the container onto the Bunsen flame and the mat.
18) Agar powder – to make agar gel.
19) Water – a medium with helps agar power to dissolve
20) Sticky tape – used to partially seal opposite ends of the agar plate.
21) Labelling equipment – pen used to make 3 quadrants on to the agar plane and their content.
22) Mass balance – to measure out 5.6 grams of agar powder.
23) Spatula – to transfer the agar powder onto the mass balance.
24) Stirring rod – to stir the agar powder and water together to make a agar solution.
Wash hands with antibacterial hand wash to kill bacteria on the surface of our hands. This reduces the risk of cross transfer of pathogens which can both interfere with the result and can infect our body.
Then wear goggles and gloves prevent the entry of bacteria into the body.
The working area must be cleaned by disinfectant before and after the...