Using Pcr And Gel Electrophoresis To Determine Genotype

772 words - 3 pages

Using PCR and Gel Electrophoresis to Determine Genotype

In certain situations, it is necessary to identify DNA retreived from a sample. When there is a
small sample in need of identification, Polymerase Chain Reactions are used to multiply the DNA
in the sample in to many identical samples. The DNA retrieved from the reaction can then be
imported into an aparatus using gel electrophoresis to compare the sample of DNA to other
samples. In our experiment we learned the how to replicate tiny samples of DNA into usable
amounts and how to analyze the specimine using gel electrophoresis. The samples of DNA were
obtained by plucking individual hairs from students' heads and using the PCR device to replicate
the DNA from the roots of the hair. The replicated DNA samples were then placed into the
electrophoresis gel and the device was turned on. Using the methods discussed above we found
that three of the fourteen samples, 21%, were homozygous and the remaining eleven samples,
79%, were heterozygous. We concluded that it is possible to examine small amounts of DNA by
first replicating the sample using Polymerase Chain Reactions then using gel electrophoresis to
determine the genotype of the DNA.

The main goal for our experiment was to learn how to examine DNA when there is only a small
sample present. We examined the samples of DNA obtained from student hair using the
replication method of Polymerase Chain Reaction and the inspection method of gel
electrophoresis. The way the PCR method works is by first mixing a solution containing the
DNA, DNA polymerase primers, and certain nucleotides. Next, the solution is heated to allow
the strands to seperate, then cooled to allow the primers in the solution to bind to the strands.
The last step occours when the DNA polymerase extends each 31 end of the primer (Campell,
1999). This process results in exact coppies of DNA molecules. The amplified samples of DNA
were placed into the gel electrophoresis aparatus where we were able to see if the sample of DNA
was heterozygous or homozygous. It is important for us to be able to replicate and examine
DNA. In the event of violent crimes, small samples such as hair, skin, seamen, blood, or urine can
give investigators a sort of genetic fingerprint of the criminal....

Find Another Essay On Using PCR and Gel Electrophoresis to Determine Genotype

A Study on Effects of Different Levels of Aloe Vera Gel as an Alternative to Antibiotics on Performance and Ileum Morphology in Broilers

1008 words - 4 pages According to the results obtained on feed intake in different periods, it can be seen that the Aloe vera groups have the highest level of feed intake compared to the control group and this leads to increased body weight gain in these groups in comparison to the control group. Olupona et al. (2010) reported increased feed intake in groups which were treated by Aloe vera gel solved in water (15, 20, 25, and 30 cm3/dm3) as body weight gain rose

The Use Of Positive And Negative Controls To Determine The Macromolecules Present In An Unknown Solution

1201 words - 5 pages The use of positive and negative controls to determine the macromolecules present in an unknown solutionCourse # 6130, Wednesday, 7-10 PM, B2-151 Lab performed: September 21st, 2011IntroductionEvery living thing is dependent on large complex molecules, known as macromolecules. The objective of this lab was to correctly identify which macromolecules the unknown solution was comprised of using various substances as experimental controls. There are

Newton's Second Law - Practical Report: To determine the relationship between force, mass and acceleration

842 words - 3 pages Aim:a:, To observe changes in motion due to changes in force and measure force, mass and acceleration, and examine their influence on motion.b: To investigate the relationship between the variables; mass, force and acceleration and determine an inertial mass through the use of a trolley, ticker-timer and set of slotted masses.Introduction:Newton's second law states "The acceleration of an object as produced by a net force is directly

How to Create Working Alliances with Business Partners, and Determine the Payoff for Each Member?

2122 words - 9 pages , outcome, and payoff. The most difficult analysis is to determine how much each player influences these factors and therefore affect the payoff of each player. Game theory gives us the tools to analyze in an accurate way these variants. Game theory revolutionized the understanding of signals by explaining communication as a strategic interaction (Maynard Smith 1974). Smith states that communications is a “strategic interaction” and from that point

Using Media to Advocate and Influence

779 words - 4 pages Media campaign is used to influence behavior of a target audience or to influence public policies. There are two types of media campaign namely; social marketing and media advocacy. Social marketing uses conventional advocating methods to influence behaviors. Media advocacy entails the use media attraction to affect pressing issues in the society. The steps to be taken when developing media campaign are: determine your objectives, determine and

Using Debt and Equity to Raise Capital

1015 words - 5 pages influence on the growth of money. The other is flexibility, sometimes known as comfort level or tolerance for risk. Considering all of these values will help make the best financial decision for raising business capital. There are basically two ways to raise business capital; using equity and using debt. To start a business using equity means you, the owner, have the financial capital/cash, or assets to get the business up and running or you can

In the following essay, the poem Beowulf is analyzed to determine if Grendel and his mother are humans or monsters

1181 words - 5 pages be morehuman than their "civilized" enemies, using violence inan act of desperation to overcome the hegemonichedonists who eschew them and treat them as anathemawhile forcing them to abscond to live as pariahs in thewilderness. Furthermore, I will show by proceedingapodictically, that Grendel and his mother have anintrinsic sense of morality and virtue that is rareamong most humans; unfortunately, the stereotypicalgeneralizations, premature

The aim of our lab was to determine the specific heat of copper and to compare with the actual result and then find the experimental error

746 words - 3 pages Specific Heat Labhttp://honolulu.hawaii.edu/distance/sci122/Programs/p21/image.gifAim:Specific heat is the amount of heat per mass required to raise the temperature of 1ºC. Different materials have different specific heats. The aim of our lab was to determine the specific heat of copper and to compare with the actual result by finding the experimental error.Materials and equipment:Electrical balanceWaterCalorimeterCylinder of

Using Gap Analysis to Understand and Improve Healthcare Delivery Practices

965 words - 4 pages Health, incoming service requests from healthcare providers are received by fax and made available to the nurse reviewers in electronic format using a computerized application. Once received by the reviewer, the nurse must access numerous web sites and programs to complete the medical necessity review process. Each of these applications is much like the standard electronic health records (EHR) utilized in many healthcare facilities. Therefore

Using AngluarJS and how to it setup - UNISA - Essay

4065 words - 17 pages ) which will be used to manage customer records. So far you have done this using ASP.NET Data Controls and utilising their various templates to coordinate viewing, editing and creating records on the same page. In this next series of practicals you will use the AngularJS JavaScript framework to achieve the same thing but on the client side. This has several advantages that you will be able to determine for yourself once the page is completed. 1

Music, Emotion and Language: Using Music to Communicate

3275 words - 13 pages Music, Emotion and Language: Using Music to Communicate ABSTRACT: There has yet to be a culture discovered which lacks music. Music is a part of our existence, but we do not fully understand it. In this paper, working in the tradition of Aristotle, Wittgenstein and Langer, I elucidate some of the connections between music and the emotions. Using contemporary philosophy of mind theories of emotion, I explain how we can have a better

Similar Essays

Ecology: Using Tracks And Signs To Determine Presence Of Mammal Species

1083 words - 5 pages Writing a short article (maximum 1000 words) on the problems and complexities of using tracks and signs to determine presence (and absence) of mammal species (25%) In the field of ecology finding and identifying tracks and signs has always been one of the hardest job in that field. This is because a range of different problems can and most likely will occur. There are several ways to find animal tracking and signs. When looking for a

Studying Photosynthetic Pigments Using Tlc Strips To Determine Evolutionary Relatedness Between Cyanobacteria, Rhodophyta, Phaeophyta, Chlorophyta And Angiosperms

953 words - 4 pages [Type text] [Type text] [Type text] Studying Photosynthetic Pigments Using TLC Strips to Determine Evolutionary Relatedness Between Cyanobacteria, Rhodophyta, Phaeophyta, Chlorophyta and AngiospermsIntroductionMany organisms around the world go through the process of photosynthesis. They include organisms such as prokaryotes and eukaryotes, specifically algae (eukaryotes) and cyanobacteria (prokaryotes). In order for eukaryotes to be able to go

Analysis Of Purified R Gfp Using Sds Page Gel/Coomassie Blue And Western Blot

2803 words - 11 pages done using SDS-PAGE gel/Coomassie Blue. Finally a Western Blot was performed to confirm the protein present is actually the protein of interest, rGFP. According to these different methods, yield, purity and rGFP activity were found and they were 4.20 ug, 15% and 10200 RFUs, repetitively.INTRODUCTIONGreen fluorescent protein, also known as GFP, is a protein produced by a jellyfish Aequorea Victoria which fluoresces in the green portion of the

Using Calorimetry To Determine The Specific Heat Of A Metal Jupiter High School Chemistry Lab Report

849 words - 4 pages I. Using Calorimetry to Determine the Specific Heat of a Metal. II. Purpose The purpose of this lab is to find the specific heat of an unknown metal by calculating the amount of energy absorbed or specific heat. That combined with prior knowledge of the principles of calorimetry. Then a comparison can be drawn to the figures of known substances on the chart provided resulting in the identity of the metal being revealed. III. Materials and