Ventilation And Oxygen Consumption Essay

481 words - 2 pages

Ventilation and Oxygen consumptionProcedure:We measured the ventillation metabolism of the fish at two different temperatures: (25 C) and (15 C). For each fish,we prepared a 250 ml jar by filling it half full with water from the aquarium in which the fish was housed.we transfered the fish from its weighing beaker to the jar adding sufficient water to now fill the jar to about 80% of its capacity.We placed the jar in the appropiate water bath. We left the jar unstoppered, and observe the activity of the fish for 10 minutes, or until its behavior stabilized. We placed a piece of sponge in the jar to help the fish stay calm. We used a two liter bottle of ice to keep the cold water tube cold.During this time, we became familiar with the ventilation pattern of the fish so that we were able to count the movements of the gill covers (#/min.). We counted the number of gill movements in 10 seconds and multiply by 6 to obtain the number of movements per minute.When we were ready to begin the experiment, we filled the jars, one at a time, with the appropriate water and allowed a small amount to overflow. Quickly we inserted the calibrated oxygen probes and take the initial oxygen readings. We took readings on each fish at 15 minute intervals for one hour. Prior to each reading, we observed and recorded the ventilation rate of the fish (#/min.) and recorded on the data.Calculation:We converted oxygen concentration readings as mg of O2 /liter of water (ppm) to ml of O2 /liter of water by dividing each value by 1.43. we then divided each...

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