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What Affect The Substrate Has On The Rate Of Reaction With The Enzyme In Potato?

946 words - 4 pages

Enzyme InvestigationTaskTo find out what affect the substrate has on the rate of reaction with the enzyme in potato.Variables1. PH2. Temperature3. Concentration of substrate4. Concentration of enzyme5. Surface area of potato lumps6. TimeKey VariablesInput variables - concentration of substrateOutput variable - volume of gasPredictionI think that to start with, the higher the concentration of the substrate the faster the rate of reaction. However, I think that eventually the graph will start to level off because the enzymes will reach the highest level at which they can work.I will keep the other variables constant to make the results more accurate and fair.Plan1. Put a set amount of potato into the conical flask.2. Then add the chosen concentration of hydrogen peroxide.3. The bubbles go through the rubber delivery tube and push water out of the measuring cylinder (the measuring cylinder is sat in a tub of water).4. We stop after a set time and measure the volume of gas.5. Repeat this process with the next concentration of the substrate (hydrogen peroxide.)SafetyI will wear safety goggles throughout and handle the hydrogen peroxide with care.ResearchAfter my preliminary tests I found that my original ideas for measurements made the reaction too fast. So now I am going to use 30 cm3 of hydrogen peroxide and 5g of potato each time. This gives a steady reaction that can be easily monitored and measured. I will be using the same amount of hydrogen peroxide each time but adding water to it, this will change the concentration.AccuracyBecause of my reaction speed with the stop watch I approximate the a few bubbles of gas may pass before I take a reading, this will only make me a few mm3 out. However, it can hopefully be avoided by careful observation. I will be repeating each concentration so an average can be taken. This should improve the accuracy of my results.ConclusionMy graph shows a curved line, which shows that concentration of hydrogen peroxide and volume of gas given off isn't directly proportional. However, the higher the concentration means the higher the volume of gas given off. This shows there is a relation between the two.In my prediction I said that the volume of gas would go up as the concentration of the substrate was increased. This was correct, as my graph shows. I also said that the line would level off because as the substrate is increased but with a constant amount of...

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