Do You Really Want Your Change Back?

2877 words - 12 pages

Davey 1Sarah DaveyCatherine Ennis11/30/2013Intro to MicrobiologyAre you sure you really want your change back?Abstract: The purpose of this experiment was to swab for an unknown bacteria. Then to use knowledge about different techniques and Medias learned about in Microbiology lab and lecture to determine the identity of the unknown bacteria. This was done by swabbing and then growing the culture on a nutrient agar plate, isolating for one bacteria, Gram staining to find morphology and gram status, taking a biochemical catalase test, Eosin Methylene Blue, Simmons Citrate, and Triple Sugar Iron Test all to select and differentiate the type of bacteria that had been isolated. Through the results of the tests applied the bacteria was determined to be Micrococcus luteus.Introduction: Aseptic technique was used to obtain the unknown bacteria, a sterile swab and gloves were used. Aseptic technique was used to avoid unwanted contaminants (Nicholson) The lab professor instructed the class to search the campus for a public used fomite. The sterile swab was saturated with distilled water then used to take a sample of bacteria from the return change drawer of a soda machine. The swab was immediately used to inoculate a Nutrient Agar plate; a general-purpose nutrient medium used for the cultivation of microbes and put in an incubator for 24 hours at 37 degrees Celsius. (Ennis, C 2013). Nutrient Agar is frequently used for isolation and purification of cultures (Nutrient Agar Plate). It consists of heat-stable digestive products of proteins (called peptones) and beef extract. (Nutrient Agar Plate) Both of these provide amino acids, minerals, and other nutrients are used by a wide variety of bacteria for optimal growth (Nutrient Agar Plate) After the bacteria was grown, an isolation streak was performed on another nutrient agar plate the following week, using a form of streaking to dilute the bacteria through four quadrants ultimately isolating one form of bacteria from the others (Ennis C. 2013). Isolation steaks are useful when it's needed to separate organisms in a mixed culture or when you need to study the colony morphology of an organism (Streak). The goal is to separate colonies into single forming units (Streak). The bacteria was once again incubated as before. Seven days later a Gram Stain, a differential staining technique was performed was used to distinguish the type of bacteria (Tortora, et al. 68). The test would show what type of gram status the "unknown bacteria was a help identify it by confirming its morphology. Materials for this test included a clean glass slide with holder, distilled water, crystal violet, iodine, alcohol, safranin, a wire loop, Pyrex dish, metal rack to set the glass slide on while staining and rinsing, a Bunsen burner, a light microscope and the isolated unknown bacteria. The Pyrex plate and rack was set up. Using the holder to balance the clean slide a small drop of distilled water was placed in the center. A...

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